| Congresso Brasileiro de Microbiologia 2023 | Resumo: 282-1 | ||||
Resumo:Salmonella spp. is an enterobacterium associated with foodborne disease outbreaks and is classified as one of the most widespread zoonotic pathogens worldwide. The control of salmonellosis represents a challenge for public health. The aim of this study was to evaluate the phenotypic profile of antimicrobial sensitivity in Salmonella spp. isolates obtained from swine feces, carcasses at different stages in the production chain, environmental samples, and final products. A total of 128 Salmonella spp. isolates were included, with 14 obtained from pre-slaughter animal feces, 51 from post-bleeding carcasses, 24 from post-scalding carcasses, 12 from post-evisceration carcasses, 1 from post-washing carcasses, 18 from environmental samples, and 8 from final products. The antimicrobial sensitivity profile was determined using the disk diffusion method following the Clinical and Laboratory Standards Institute (CLSI, 2020) guidelines, using the following antimicrobials: gentamicin 10 μg, streptomycin 10 μg, imipenem 10 μg, meropenem 10 μg, ceftazidime 30 μg, cefoxitin 30 μg, ciprofloxacin 5 μg, norfloxacin 10 μg, trimethoprim-sulfamethoxazole 25 μg, ampicillin 10 μg, chloramphenicol 30 μg, tetracycline 30 μg, and nitrofurantoin 300 μg. The highest rates of resistance were observed for chloramphenicol 65.62% (84/128), ampicillin and tetracycline 60.94% (78/128), streptomycin 37.50% (48/128), gentamicin and nitrofurantoin 2.34% (3/128). No isolates had resistance to meropenem, ceftazidime, ceftiofur, cefoxitin, norfloxacin, and sulfamethoxazole-trimethoprim. In total, 57% (73/128) of the isolates exhibited multidrug resistance, with 41.09% (30/73) displaying resistance to ampicillin-chloramphenicol-tetracycline, 31.50% (23/73) to streptomycin-ampicillin-chloramphenicol-tetracycline, 24.65% (18/73) to streptomycin-ampicillin-tetracycline, and 2.73% (2/73) to gentamicin-streptomycin-ampicillin-chloramphenicol-tetracycline. Thirty multidrug-resistant isolates (41.09%) were obtained from the post-bleeding, 30.13% (22/73) from the post-scalding, 10.95% (8/73) from environmental samples, 9.58% (7/73) from the post-evisceration, 4.10% (3/73) from pre-slaughter animal feces, and from final products. Factors favoring the emergence of antimicrobial resistance are related to the irresponsible use of drugs in humans and the use of antimicrobials to prevent diseases in healthy animals and as growth promoters, contributing to the spread of resistant bacteria in the animal production chain. It is evident the need for monitoring multidrug-resistant microorganisms in the production chain, especially Salmonella spp. Palavras-chave: food, multidrug resistance, pathogens, public health, pork Agência de fomento:Conselho Nacional de Desenvolvimento Científico e Tecnológico – (CNPq). | |||||